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Genetic recombination for hGH synthesis and the removal of introns?
When manufacturing hGH a sample of mRNA from the hypothalamus is converted by reverse transcriptase to cDNA for incorporation into a bacterial plasmid. However bacterial cells dont have introns. Is it the primary transcript or processed mRNA that is acted on by reverse transcriptase to form the cDNA
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- The mRNA transcripts from the hypothalamus are processed transcripts with their introns are deleted. So we use reverse transcriptase to convert the hGH mRNA to cDNA that represent the gene of hGH but without introns ( just exons). Because bacterial cells don't have introns we use cDNA to introduce foreign genes into bacterial cells to produce recombinant products.
- It is the processed mRNA. The whole point of cDNA is that the introns have been removed. If you wanted to then sequence the cDNA you would be able to get at just the coding sequence without spending a lot of time and money obtaining kilobases of intron sequence, which is what would happen if you were dealing with genomic DNA.
